کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
21106 43206 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Direct measurement of oxygen concentration inside cultured cartilage for relating to spatial growth of rabbit chondrocytes
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Direct measurement of oxygen concentration inside cultured cartilage for relating to spatial growth of rabbit chondrocytes
چکیده انگلیسی

In static culture of rabbit chondrocytes in collagen gel, the direct measurement of dissolved oxygen (DO) concentration revealed that the DO level at the top surface of gel decreased due to an increase in overall cell density with elapsed time. The local cell density at the top surface on day 21 was 5.7 × 107 cells/cm3, being 11 times that at the bottom of gel. This heterogeneity of cell distribution in the gel was considered to occur by limitation of oxygen supply into a deeper part of the gel. In shaking culture using a dish with gas-permeable film, the DO level was enhanced inside the gel and the overall cell density in the gel was achieved to be 2.9 times that in the static culture.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 3, September 2010, Pages 363–366
نویسندگان
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