کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2119476 1085394 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Single-cell level analysis of megakaryocyte growth and development
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Single-cell level analysis of megakaryocyte growth and development
چکیده انگلیسی

Several fundamental questions regarding cell growth and development can be answered by recording and analyzing the history of cells and their progeny. Herein, long-term and large-field live cell imaging was used to study the process of megakaryopoiesis at the single cell level (n=9300) from human CD34+ cord blood (CB) in the presence of thrombopoietin (TPO) or the cytokine cocktail BS1 with or without nicotinamide (NIC). Comparative analyses revealed that the cocktail BS1 increased the mitotic and proplatelet rate of diploid and polyploid cells, respectively. Conversely, only NIC treatment increased the endomitotic rate of megakaryocytes (MKs) leading to the formation of CB-MKs with ploidy level frequently observed with BM-MKs. However, NIC failed to enhance platelet production. Rather, a 7- and 31-fold reduction in proplatelet formation was observed in tetraploid and octaploid CB-MKs, respectively, and ex vivo platelet production output was reduced by half due to a reduction in MK output in NIC cultures. Unexpectedly, a significant fraction of di- and polyploid CB-MKs were seen to undergo complete proplatelet regression. Though rare (<0.6%), proplatelet reversal led to the formation of regular round cells that could at times resume normal development. The cell tracking data was then used to investigate the impact of “developmental fate” and ploidy on cell cycling time, and to identify potential developmental patterns. These analyses revealed that cell fate and ploidy level have major impacts on the cell cycling time of the cells, and that four recurrent cell lineage patterns could be identified for CD34+ cells undergoing MK differentiation.


► Long term live cell imaging was used to study the developmental and fate of megakaryocytes.
► Nicotinamide raised the ploidy of cord blood megakaryocytes but not platelet production.
► The fate and ploidy level of the cells have major impacts on the cell cycling time.
► Sightings of complete proplatelet regression are also reported.
► Four recurrent megakaryocyte differentiation cell lineage patterns were identified.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Differentiation - Volume 83, Issue 4, April 2012, Pages 200–209
نویسندگان
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