کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
21370 | 43219 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide](/preview/png/21370.png)
We report that several tryptophan-rich peptides exhibit an affinity for a hydrophobic ionic liquid (IL) (1-ethyl-3-methylimidazolium bis-trifluoromethanesulfonyl imide), and that green fluorescent protein (GFP) fused to a peptides, “SSSWWSWWWW” (SW1) or “SWWWWSWWWW” (SW2), containing serine (S) and tryptophan (W) at the C terminus localized at the IL/water interface. While GFPs without W-rich peptide distributed only in water phase, SW1- and SW2-GFPs were accumulated at the interface. The localization of SW1-GFP showed biphasic behavior, and most distinctive localization was observed at 7.1 μM. The localization of SW2-GFP presumably occurred at largely lower concentration (≤ 0.5 μM) than that of SW1-GFP, which difference was due to the higher hydrophobicity of SW2 peptide.
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 2, February 2012, Pages 160–165