کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2143767 | 1088358 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification and validation of S100A7 associated with lung squamous cell carcinoma metastasis to brain
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کلمات کلیدی
S100A7SCCPMFMS/MS - MS / MSAdenocarcinoma - آدنوکارسینوماPeptide mass fingerprint - اثر انگشت پپتیدهTwo-dimensional electrophoresis - الکتروفورز دو بعدیIHC - ایمونوهیستوشیمیImmunohistochemistry - ایمونوهیستوشیمیTandem MS - تاندوم MSNSCLC - سرطان ریوی غیر سلول کوچکNon-small cell lung cancer - سرطان غیر سلول کوچک ریهMass spectrometry - طیف سنجی جرمیMALDI-TOF MS - مالدی توف MSBrain metastasis - متاستاز مغزیMatrix-assisted laser desorption/ionization time of flight mass spectrometry - مدت زمان جذب / زمان یونیزاسیون طیف سنجی جرمی پرواز با کمک ماتریکسSquamous cell carcinoma - کارسینوم سلول سنگفرشی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To identify potential markers associated with non-small cell lung cancer (NSCLC) metastasis to brain, comparative proteome analysis on two lung squamous cell carcinoma (SCC) cell lines, NCI-H226 and H226Br (the brain metastatic cell line of NCI-H226), was performed using two-dimensional electrophoresis (2-DE) followed by a tandem mass spectrometer with a matrix-assisted laser desorption/ionization (MALDI) source. Twenty differential proteins were identified, of which 6 proteins were up-regulated in H226Br cell compared with NCI-H226 cells, whereas 14 proteins were down-regulated. S100A7 and 14-3-3Ï, two of candidate proteins significantly upregulated and downregulated in H226Br cell, were selected to verify the liability of the differential proteins by Western blot. The results were in accordance with 2-D data. To determine whether S100A7 overexpression is actually associated with SCC metastasis to brain, S100A7 protein was testified in 10 brain metastasis tissues from NSCLC, 38 primary NSCLC tissues including half matched local positive lymph nodes, 5 primary brain tumors and 2 non-cancer brain tissues by immunohistochemistry. Of particular interest to us was that the positive staining of S100A7 could be found in 3/5 (60%) brain metastases tissue from SCC and 8/21 (38%) the primary lung SCC tissues, while no positive staining was observed in the brain metastases tissue from Ad (n = 5), the primary adenocarcinoma (Ad) tissues (n = 17), the primary brain tumors (n = 5), all local positive lymph nodes from the primary NSCLC (n = 19) and non-cancer brain tissues (n = 2). These findings suggest that S100A7 expression is closely associated with SCC metastasis to brain and may be a potential biomarker for monitoring the development of SCC.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Lung Cancer - Volume 57, Issue 1, July 2007, Pages 37-45
Journal: Lung Cancer - Volume 57, Issue 1, July 2007, Pages 37-45
نویسندگان
Hao Zhang, Yinping Wang, Yue Chen, Suozhu Sun, Na Li, Dongxia Lv, Chuanjun Liu, Lingyun Huang, Dacheng He, Xueyuan Xiao,