کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2144873 | 1088631 | 2012 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hyaluronan and versican in the control of human T-lymphocyte adhesion and migration
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ECMPBMCnTregVersicanpolyinosine-polycytidylic acidbiotinylated hyaluronan binding proteininflammation - التهاب( توروم) Peripheral blood mononuclear cell - سلول تک هسته ای خون محیطیendoplasmic reticulum - شبکه آندوپلاسمی Human lung fibroblasts - فیبروبلاست های انسانی ریهLymphocyte - لنفوسیتExtracellular matrix - ماتریکس خارج سلولیMigration - مهاجرتMyofibroblast - میوفیبروبلاستHyaluronan - هیالورونانPoly I:C - پلی I: C
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The ability of lymphocytes to migrate freely through connective tissues is vital to efficient immune function. How the extracellular matrix (ECM) may affect T-cell adhesion and migration is not well understood. We have examined the adhesion and migration of activated human T-lymphocytes on ECM made by fibroblast-like synoviocytes and lung fibroblasts. These cells were minimally interactive until treated with a viral mimetic, Poly I:C. This treatment promoted myofibroblast formation and engendered a higher-order structured ECM, rich in versican and hyaluronan, to which T-cells avidly adhered in a hyaluronidase-sensitive manner. This Poly I:C-induced matrix impeded T-cell spreading and migration on and through synoviocyte monolayers, while hyaluronidase treatment or adding versican antibody during matrix formation reversed the effect on T-cell migration. Hyaluronidase also reversed the spread myofibroblast morphology. These data suggest that the viscous hyaluronan- and versican-rich matrix binds and constrains T-lymphocytes. Using purified matrix components and solid state matrices of defined composition, we uncovered a role for versican in modulating hyaluronan-T-cell interactions. Versican prevented T-cell binding to soluble hyaluronan, as well as the amoeboid shape change on hyaluronan-coated dishes and T-cell penetration of collagen gels. Together, these data suggest that hyaluronan and versican play a role in T-cell trafficking and function in inflamed tissues.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Matrix Biology - Volume 31, Issue 2, March 2012, Pages 90-100
Journal: Matrix Biology - Volume 31, Issue 2, March 2012, Pages 90-100
نویسندگان
Stephen P. Evanko, Susan Potter-Perigo, Paul L. Bollyky, Gerald T. Nepom, Thomas N. Wight,