Experimental measurement and modelling of solubility of inosine-5′-monophosphate disodium in pure and mixed solvents

• Solubility of 5′-IMPNa2 in various solvents was studied for the first time.
• The solubility could be ranked as follows: water > methanol > ethanol > acetone.
• Modified Apelblat equation gave the best correlating results.
• Mixing Gibbs free energies, enthalpies, and entropies were predicted.
• Solubility data and equations can optimise the crystallization conditions.

The solubility of biological chemicals in solvents provide important fundamental data and is generally considered as an essential factor in the design of crystallization processes. The equilibrium solubility data of inosine-5′-monophosphate disodium (5′-IMPNa2) in water, methanol, ethanol, acetone, as well as in the solvent mixtures (methanol + water, ethanol + water, acetone + water), were measured by an isothermal method at temperatures ranging from (293.15 to 313.15) K. The measured data in pure and mixed solvents were then modelled using the modified Apelblat equation, van’t Hoff equation, λh equation, ideal model and the Wilson model. The modified Apelblat equation showed the best modelling results, and it was therefore used to predict the mixing Gibbs free energies, enthalpies, and entropies of 5′-IMPNa2in pure and binary solvents. The positive values of the calculated partial molar Gibbs free energies indicated the variations in the solubility trends of 5′-IMPNa2. Water and ethanol (in the binary mixture with water) were found to be the most effective solvent and anti-solvent, respectively.

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