کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
216183 1426266 2012 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Study on the thermodynamics of the binding of iminium and alkanolamine forms of the anticancer agent sanguinarine to human serum albumin
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Study on the thermodynamics of the binding of iminium and alkanolamine forms of the anticancer agent sanguinarine to human serum albumin
چکیده انگلیسی

Sanguinarine is an anticancer plant alkaloid that can exist in the charged iminium and neutral alkanolamine forms. The thermodynamics of the interaction of the two forms with human serum albumin was investigated using calorimetric techniques, and the data supplemented with circular dichroism and spectrofluorimetric studies. The thermodynamic results show that there is only one class of binding for sanguinarine on HSA. The equilibrium constant was four times higher for the alkanolamine (Ka = 2.18 · 105 M−1) than for iminium (Ka = 5.97 · 104 M−1). The binding was enthalpy driven for iminium and favoured by both a negative enthalpy and a stronger favourable entropy contribution for the alkanolamine. Temperature dependent calorimetric data yielded values of ΔCp∘ that are consistent with the involvement of different molecular forces in the complexation of the two forms of sanguinarine to HSA. The fluorescence quenching data suggest a static quenching mechanism. Synchronous fluorescence and circular dichroic data are consistent with a conformational change in the protein on binding that was also higher for the alkanolamine form.


► Energetics of sanguinarine–human serum albumin has been elucidated.
► The alkanolamine binds stronger than iminium.
► Enthalpy driven binding for iminium was revealed.
► Alkanolamine form binding was favored by negative enthalpy and entropy changes.
► Spectroscopic results support calorimetry data.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Chemical Thermodynamics - Volume 47, April 2012, Pages 90–99
نویسندگان
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