کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2166705 | 1549326 | 2006 | 13 صفحه PDF | دانلود رایگان |
Nutrient-induced increases in intracellular free Ca2+ concentrations are the key trigger for insulin release from pancreatic islet β-cells. These Ca2+ changes are tightly regulated temporally, occurring as Ca2+ influx-dependent baseline oscillations. We explore here the concept that locally high [Ca2+] concentrations (i.e. Ca2+ microdomains) may control exocytosis via the recruitment of key effector proteins to sites of exocytosis. Importantly, recent advances in the development of organelle- and membrane-targeted green fluorescent protein (GFP-) or aequorin-based Ca2+ indicators, as well as in rapid imaging techniques, are providing new insights into the potential role of these Ca2+ microdomains in β-cells. We summarise here some of the evidence indicating that Ca2+ microdomains beneath the plasma membrane and at the surface of large dense core vesicles may be important in the normal regulation of insulin secretion, and may conceivably contribute to “ATP-sensitive K+-channel independent” effects of glucose. We also discuss evidence that, in contrast to certain non-excitable cells, direct transfer of Ca2+ from the ER to mitochondria via localised physical contacts between these organelles is relatively less important for efficient mitochondrial Ca2+ uptake in β-cells. Finally, we discuss evidence from single cell imaging that increases in cytosolic Ca2+ are not required for the upstroke of oscillations in mitochondrial redox state, but may underlie the reoxidation process.
Journal: Cell Calcium - Volume 40, Issues 5–6, November–December 2006, Pages 539–551