کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2167820 | 1549419 | 2009 | 7 صفحه PDF | دانلود رایگان |

IFN-γ has significant immunoregulatory activity and plays an important role in both innate and adaptive immunity. Additive effects of IFN-γ and the Toll-like receptor ligand LPS has been investigated in macrophages, but in fibroblasts is incompletely understood. IFN-γ and LPS synergistically induced MCP-1 and NO release in primary murine dermal fibroblasts. IFN-γ enhanced LPS-induced JNK and p38 MAPK phosphorylation but had no effect on NF-κB activity. The induction of both MCP-1 and NO was attenuated by inhibition of JNK but not p38 MAPK. Serine 727 STAT1 phosphorylation by IFN-γ was increased by LPS, and this was also attenuated by inhibition of JNK but not p38 MAPK. IFN-γ inhibited the basal expression of MAPK phosphatase-1, a negative regulator of MAPK signaling pathway. These results suggest that enhancement of LPS-induced JNK activation by IFN-γ associated with inhibition of MAPK phosphatase-1 may be one of the mechanisms of additive effects between IFN-γ and LPS in fibroblasts.
Journal: Cellular Immunology - Volume 255, Issues 1–2, 2009, Pages 26–32