کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2168806 1092917 2010 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Curve fitting approach for measurement of cellular osmotic properties by the electrical sensing zone method. II. Membrane water permeability
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Curve fitting approach for measurement of cellular osmotic properties by the electrical sensing zone method. II. Membrane water permeability
چکیده انگلیسی

In a companion paper, we demonstrated that dynamic range limitations can confound measurement of the osmotically inactive volume using electrical sensing zone instruments (e.g., Coulter counters), and presented an improved parameter estimation method in which a lognormal function was fit to the cell volume distribution to allow extrapolation beyond the bounds of the data. Presently, we have investigated the effect of dynamic range limitations on measurement of the cell membrane water permeability (Lp), and adapted the lognormal extrapolation method for estimation of Lp from transient volume data. An alternative strategy (the volume limit adjustment method, in which the measured isotonic volume distribution is used to generate model predictions for curve fitting, and the bounds of the dynamic range are adjusted such that extrapolation is not required) was also developed. The performance of these new algorithms was compared to that of a conventional parameter estimation method. The best-fit Lp values from in vitro experiments with mouse insulinoma (MIN6) cells differed significantly for the different parameter estimation techniques (p < 0.001). Using in silico experiments, the volume limit adjustment method was shown to be the most accurate (relative error 0.4 ± 3.2%), whereas the conventional method underestimated Lp by 19 ± 2% for MIN6 cells. Parametric analysis revealed that the error associated with the conventional method was sensitive to the dynamic range and the width of the volume distribution. Our initial implementation of the lognormal extrapolation method also yielded significant errors, whereas accuracy of this algorithm improved after including a normalization scheme.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 60, Issue 2, April 2010, Pages 117–128
نویسندگان
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