کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2168902 1092921 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cryo-responses of two types of large unilamellar vesicles in the presence of non-permeable or permeable cryoprotecting agents
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Cryo-responses of two types of large unilamellar vesicles in the presence of non-permeable or permeable cryoprotecting agents
چکیده انگلیسی

Cryo-responses of two types of large unilamellar vesicles (LUV) that were made from either egg yolk l-α-phosphatidylcholine (EPC) or 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (DPPC), in the presence of non-permeable or permeable cryoprotective agents (CPA) was investigated. Partial ternary phase diagrams of CPA–salt–water with specific CPA to salt ratio (R), were constructed to estimate the phase volume of ice and unfrozen matrix of the LUV dispersion, which could aid in understanding the mechanistic actions of CPA. Leakage of both EPC and DPPC LUV was reduced if the sugar concentrations are above 10% (w/w) for disaccharides and 5% (w/w) for monosaccharides. Above these sugar concentrations, non-permeable CPA were more effective in preventing leakage of DPPC LUV than in EPC LUV. Below these sugar concentrations, EPC and DPPC LUV with limited mobility in the remaining unfrozen matrix were more likely to approach and interact with one and another, which were not anticipated when the LUV were completely embedded in the ice matrix. In the presence of Me2SO or EG, EPC LUV that had been subjected to freezing and thawing processes were protected from leakage. At room temperature, Me2SO and EG were detrimental to the DPPC LUV. This study suggests that the choice of CPA for cell cryopreservation depends on the type of phospholipids in plasma membranes, which vary in their acyl chain length and gel–liquid crystal phase transition temperature.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 57, Issue 3, December 2008, Pages 276–285
نویسندگان
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