کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2172374 1093538 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of dextran on proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Effects of dextran on proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells
چکیده انگلیسی

BackgroundBioreactors have been used extensively to construct tissue-engineered bone. However, mass transport and flow shear stress, the two main factors contributing to the construction of tissue-engineered bone, cannot be studied separately when changing the perfusing rate. A tackifier needs to be added into the medium to separate the two factors. This study investigated the feasibility of using dextran as a tackifier in the study of individual effects of mass transport and flow shear stress on the construction of tissue-engineered bone.MethodsHuman bone marrow-derived mesenchymal stromal cells (hBMSC) were isolated and cultured in media containing 1%, 3%, 5%, 7% and 9% dextran [molecular weight (MW) 70000]. Cell proliferation and osteogenic differentiation were observed in vitro. hBMSC cultured without dextran provided a control group.ResultsCell proliferation was found to have no significant statistical difference (P > 0.05) with a dextran concentration range of 1–7%. No significant difference in alkaline phosphatase (ALP) activity (P > 0.05) was found among the different groups. Alizarin red staining showed no difference in mineralized extracellular matrix among the groups with a concentration of dextran less than 9%. Expression of collagen, ALP, osteopontin (OPN) and osteocalcin (OC) genes did not show any difference with a concentration range of 1–7%.DiscussionWhen using a bioreactor to construct tissue-engineered bone, it is feasible to add a 1–7% dextran (MW 70000) concentration into the medium in order to study the effects of mass transport and flow shear stress.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cytotherapy - Volume 10, Issue 6, 2008, Pages 587–596
نویسندگان
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