کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
21745 43238 2009 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of non-B DNA sequences on transgene expression
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Effects of non-B DNA sequences on transgene expression
چکیده انگلیسی

DNA conformation may be an important factor affecting gene transcription. In this study, we examined how DNA sequences with unusual conformations affect transgene expression. A30 and (CG)15 sequences that can adopt the B′ and Z conformations, respectively, were introduced into a β-actin promoter. Luciferase plasmids containing the manipulated promoter were transfected into NIH3T3 cells by electroporation and were delivered into mouse livers with a hydrodynamics-based injection. Expression from plasmid with the (CG)15 sequence was multiple times higher than expression from control plasmid DNA. The A30 sequence also tended to enhance expression. These results suggest that non-B DNA sequences could improve transgene expression in cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 108, Issue 1, July 2009, Pages 20–23
نویسندگان
, , ,