کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2179394 1549942 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of genetic variability in Aquilaria malaccensis from Bramhaputra valley, Assam, India using ISSR markers
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
Analysis of genetic variability in Aquilaria malaccensis from Bramhaputra valley, Assam, India using ISSR markers
چکیده انگلیسی


• The study showed high intra population diversity indicating genetic fitness of A. malaccensis from Northeast India.
• The study revealed that the majority of variation existed within populations rather than among populations.
• The pattern of genetic diversity observed in A. malaccensis indicated existence of diverse array of genotypes from Northeast India.

Aquilaria malaccensis is a threatened agarwood producing plant with high ecological and economic importance. In the present study, we used inter-simple sequence repeat (ISSR) markers to analyze the pattern of genetic variation and differentiation among seven populations comprising 53 individuals of the species, dispersed across the biodiversity rich Brahmaputra valley of Assam, India. Of the 50 ISSR primers initially evaluated, 16 primers generated distinct and reproducible DNA amplicons. Using these 16 primers, 166 discernible DNA amplicons were generated of which 155 (93.4%) were polymorphic. Total genotype diversity among the populations (HT) was 0.25 (0.03), while the within population diversity (HS) was 0.14 (0.02). The mean coefficient of gene differentiation (GST) was as high as 0.44, resulting in an estimate for gene flow (Nm) of 0.63, indicating moderate genetic differentiation among the populations of A. malaccensis. Analysis of molecular variance (AMOVA) indicated that 38% variance was attributable to among-populations diversity and the rest (62%) to differences within populations. The pattern of genetic diversity observed in A. malaccensis indicated existence of a diverse array of genotypes, implicating the need for preservation of every population to maintain genetic variability in this highly important and vulnerable tree species.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Flora - Morphology, Distribution, Functional Ecology of Plants - Volume 217, November 2015, Pages 24–32
نویسندگان
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