کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2181315 1095288 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improved tryprostatin B production by heterologous gene expression in Aspergillus nidulans
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Improved tryprostatin B production by heterologous gene expression in Aspergillus nidulans
چکیده انگلیسی

Tryprostatin B, a prenylated diketopiperazine with anti-tubulin activity, has been overproduced in fungal culture by expression of genes of the fumitremorgin cluster from Aspergillus fumigatus in the naïve host Aspergillus nidulans using the alcA promoter. The products of the expressed genes catalyse the first two steps of fumitremorgin biosynthesis, namely the formation of brevianamide F and its conversion to tryprostatin B. Yields of tryprostatin B were up to 250 mg/l, a significant improvement in previously reported levels. This approach illustrates how the availability of fungal genome sequences and knowledge of gene function can be used to achieve the efficient production of biologically active secondary metabolites by genetic manipulation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 46, Issue 5, May 2009, Pages 436–440
نویسندگان
, , , ,