کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2181515 | 1095301 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Evidence of RIP (repeat-induced point mutation) in transposase sequences of Aspergillus oryzae Evidence of RIP (repeat-induced point mutation) in transposase sequences of Aspergillus oryzae](/preview/png/2181515.png)
A DNA methyl-binding column was used to isolate genomic fragments enriched for DNA-methylation from Aspergillus parasiticus. One of the isolated sequences presented 67% identity at the protein level with the transposase from the transposable element Tan1 of Aspergillus niger var. awamori, and was found to be present in at least 20 copies in the Aspergillus oryzae database. Analysis of four copies showed evidence of C:G to T:A transitions in at least 98.2% of the mutations found over a 1032–1180 bp region spanning a large part of the transposase sequence. Using copy specific primers three sequences were amplified from a different strain of A. oryzae and a similar pattern of C:G to T:A transitions was found. These transitions are similar to those observed in RIP, in Neurospora crassa, where cytosine-methylation is believed to be involved. Using methylation-sensitive Southern blotting, no evidence of methylation was found in the transposase sequences in these two A. oryzae strains as well as one A. parasiticus and one Aspergillus flavus strain.
Journal: Fungal Genetics and Biology - Volume 43, Issue 6, June 2006, Pages 439–445