کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2181984 1550060 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation of knockout mice expressing a GFP-reporter under the control of the Lmx1a locus
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Generation of knockout mice expressing a GFP-reporter under the control of the Lmx1a locus
چکیده انگلیسی

Lmx1a is a member of the LIM homeodomain containing transcription factors and plays an important role during embryonic development. Specifically, it is required for the proper formation of several structures in the central nervous system, such as the roof plate, the cerebellum, and the inner ear. All these defects may contribute to the neurological phenotype observed in dreher mice, lacking functional Lmx1a protein. Interestingly, this factor was also found to promote midbrain dopaminergic neuron fate. We have introduced Green fluorescent protein (GFP) coding sequences into the Lmx1a locus by homologous recombination, and created knockout mice where GFP recapitulates the Lmx1a endogenous expression pattern.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Expression Patterns - Volume 11, Issues 5–6, June–July 2011, Pages 345–348
نویسندگان
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