Zebrafish transgenic lines co-expressing a hybrid Gal4 activator and eGFP in tissue-restricted patterns

► Description of 16 transgenic zebrafish lines expressing eGFP and a hybrid Gal4 activator in tissue-specific patterns. ► Transgenic lines showing specific expression patterns in retina, blood vessels and hematopoietic cells. ► Gal4/UAS mediated transactivation makes the transgenic lines useful as genetic tools to study developmental processes. ► Mapping of genomic positions of activated insertions identifies three endogenous genes expressed in retina. ► An identified RGM-containing gene expressed in ventral retina may have a role in axonal guidance.