کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
218204 463188 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enhanced electrochemical biosensing efficiency of silica particles supported on partially reduced graphene oxide for sensitive detection of cholesterol
ترجمه فارسی عنوان
افزایش کارایی ذرات سیلیس در آزمایشهای بیوشیمیایی الکتروشیمیایی بر کاهش میزان اکسید گرافین به میزان قابل توجهی برای تشخیص حساسیت کلسترول
کلمات کلیدی
ذرات سیلیس، به طور جزئی کاهش اکسید گرافین، حساسیت کلسترول، ولتامتری سیسیک، مقدار کیلومتر
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی


• Thin films of pRGO–SiO2 particles hybrid system (pRGOSHs) have been fabricated.
• ChOx/pRGO/ITO and ChOx/pRGOSHs/ITO were utilized for cholesterol biosensing.
• Sensitivity is of 11.1 μA/mM/cm2 observed for the ChOx/pRGOSHs/ITO biosensor.
• Lower km values for ChOx-pRGOSHs/ITO (4.9 × 10–4 mM) were observed.
• EIS shows low impedance values for pRGOSHs electrode.

The present work introduces partially reduced graphene oxide (pRGO)-silica (SiO2) particles hybrid system (pRGOSHs) for sensitive and cost effective free cholesterol detection. Fabricated out of thin layers of pRGOSHs, these proposed ChOx/pRGOSHs/ITO based biosensors have a detection range of 2.6–15.5 mM with an appreciable detection limit of 1.3 mM and sensitivity of 11.1 μA/mM/cm2. Low Michaelis–Menten constant (Km) (4.9 × 10− 4 mM) and high diffusivity constant (D) (3.2 × 10− 10 cm2/s) values clearly indicate enhanced immobilization of enzyme over the substrate. Additionally, electrochemical impedance studies indicate that the synergistic combination of SiO2 and pRGO also results in much lower impedance values (40% and 18% decrease in comparison to SiO2 and pRGO respectively) for an overall enhanced sensing performance. These results are further corroborated by the density functional theory based theoretical simulations indicating enhanced electron density (theoretically) in case of the proposed hybrid system. Finally, the present work also highlights the importance of Si–OH bonds formation in the proposed pRGOSHs composite system for attaining such enhanced biosensing ability.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Electroanalytical Chemistry - Volume 757, 15 November 2015, Pages 65–72
نویسندگان
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