کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2190244 | 1096248 | 2006 | 13 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Roles of N-terminal Pyroglutamate in Maintaining Structural Integrity and pKa Values of Catalytic Histidine Residues in Bullfrog Ribonuclease 3 Roles of N-terminal Pyroglutamate in Maintaining Structural Integrity and pKa Values of Catalytic Histidine Residues in Bullfrog Ribonuclease 3](/preview/png/2190244.png)
Many proteins and bioactive peptides contain an N-terminal pyroglutamate residue (Pyr1). This residue reduces the susceptibility of the protein to aminopeptidases and often has important functional roles. The antitumor ribonuclease RC-RNase 3 (RNase 3) from oocytes of Rana catesbeiana (bullfrog) is one such protein. We have produced recombinant RNase 3 containing the N-terminal Pyr1 (pRNase 3) and found it to be indistinguishable from the native RNase 3 by mass spectrometry and a variety of other biochemical and immunological criteria. We demonstrated by NMR analysis that the Pyr1 of pRNase 3 forms hydrogen bonds with Lys9 and Ile96 and stabilizes the N-terminal α-helix in a rigid conformation. In contrast, the N-terminal α-helix becomes flexible and the pKa values of the catalytic residues His10 and His97 altered when Pyr1 formation is blocked by an extra methionine at the N terminus in the recombinant mqRNase 3. Thus, our results provide a mechanistic explanation on the essential role of Pyr1 in maintaining the structural integrity, especially at the N-terminal α-helix, and in providing the proper environment for the ionization of His10 and His97 residues for catalysis and cytotoxicity against HeLa cells.
Journal: Journal of Molecular Biology - Volume 355, Issue 3, 20 January 2006, Pages 409–421