Analysis of Selective Production of Nα-Benzyloxycarbonyl-l-Aminoadipate-δ-Semialdehyde and Nα-Benzyloxycarbonyl-l-Aminoadipic Acid by Rhodococcus sp. AIU Z-35-1

The factors for selective production of Nα-benzyloxycarbonyl-l-aminoadipate-δ-semialdehyde (Nα-Z-l-AASA) and Nα-benzyloxycarbonyl-l-aminoadipic acid (Nα-Z-l-AAA) from Nα-benzyloxycarbonyl-l-lysine (Nα-Z-l-lysine) by Rhodococcus sp. AIU Z-35-1 were analyzed. The cultivation time was important for selective production of Nα-Z-l-AASA, since Nα-Z-l-lysine oxidizing enzyme reached maximum at the early stage of cell growth and then decreased. The differences of enzyme activities of Nα-Z-l-lysine oxidizing enzyme and Nα-Z-l-AASA dehydrogenase in pH and temperature also affected the selective production of Nα-Z-l-AASA. For efficient production of Nα-Z-l-AAA, it was important for cultivation time that Nα-Z-l-AASA dehydrogenase activity be higher than Nα-Z-l-lysine oxidizing enzyme activity, since a high concentration of Nα-Z-l-AASA inhibited Nα-Z-l-AASA dehydrogenase activity. The optimum pH of Nα-Z-l-AAA production was affected by the instability of Nα-Z-l-AASA dehydrogenase in the alkaline pH region.