The long N-terminus of the human monocarboxylate transporter 8 is a target of ubiquitin-dependent proteasomal degradation which regulates protein expression and oligomerization capacity

• The long N-terminus of MCT8 enhances proteasomal degradation.
• The long N-terminus of MCT8 is a target of ubiquitin conjugation.
• Proteasomal degradation affects MCT8 amount and capacity for oligomerization.

Monocarboxylate transporter 8 (MCT8) equilibrates thyroid hormones between the extra- and the intracellular sides. MCT8 exists either with a short or a long N-terminus, but potential functional differences between both variants are yet not known.We, therefore, generated MCT8 constructs which are different in N-terminal length: MCT8(1–613), MCT8(25–613), MCT8(49–613) and MCT8(75–613). The M75G substitution prevents translation of MCT8(75–613) and ensures expression of full-length MCT8 protein. The K56G substitution was made to prevent ubiquitinylation. Cell-surface expression, localization and proteasomal degradation were investigated using C-terminally GFP-tagged MCT8 constructs (HEK293 and MDCK1 cells) and oligomerization capacity was determined using N-terminally HA- and C-terminally FLAG-tagged MCT8 constructs (COS7 cells).MCT8(1–613)-GFP showed a lower protein expression than the shorter MCT8(75–613)-GFP protein. The proteasome inhibitor lactacystin increased MCT8(1–613)-GFP protein amount, suggesting proteasomal degradation of MCT8 with the long N-terminus. Ubiquitin conjugation of MCT8(1–613)-GFP was found by immuno-precipitation. A diminished ubiquitin conjugation caused by K56G substitution resulted in increased MCT8(1–613)-GFP protein expression. Sandwich ELISA was performed to investigate if the bands at higher molecular weight observed in Western blot analysis are due to MCT8 oligomerization, which was indeed shown.Our data imply a role of the long N-terminus of MCT8 as target of ubiquitin-dependent proteasomal degradation affecting MCT8 amount and subsequently oligomerization capacity.