کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2196106 1550891 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
D-chiro-inositol glycan stimulates insulin secretion in pancreatic β cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
D-chiro-inositol glycan stimulates insulin secretion in pancreatic β cells
چکیده انگلیسی


• INS-2 is a D-chirl-inositol with insulin mimicking effect.
• INS-2 stimulates insulin secretion from pancreatic β cells.
• INS-2 stimulates insulin release by inhibiting ATP-sensitive K+ channels (KATP).
• The inhibitory effect of INS-2 on KATP is mediated by protein phosphatase 2C.

Insulin has been shown to act on pancreatic β cells to regulate its own secretion. Currently the mechanism underlying this effect is unclear. INS-2, a novel inositol glycan pseudo-disaccharide containing D-chiro-inositol and galactosamine, has been shown to function as an insulin mimetic and a putative insulin mediator. In the present study we found that INS-2 stimulates insulin secretion in MIN6 β cells and potentiates glucose stimulated insulin secretion in isolated mouse islets. Importantly, INS-2 failed to potentiate insulin secretion induced by tolbutamide, which stimulates insulin release by closing ATP sensitive potassium channels (KATP). Electrophysiological studies showed that INS-2 inhibited sulfonylurea-sensitive KATP conductance. The effect of INS-2 on inhibiting KATP channel is mediated by protein phosphatase 2C (PP2C), as knocking down PP2C expression in MIN6 cells by PP2C small hairpin RNA completely abolished the effect of INS-2 on KATP and consequently attenuated INS-2 induced insulin secretion. In conclusion, the present study identifies a novel mechanism involving PP2C in regulating KATP channel activity and consequently insulin secretion.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 387, Issues 1–2, 25 April 2014, Pages 1–7
نویسندگان
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