کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2196155 | 1550899 | 2013 | 7 صفحه PDF | دانلود رایگان |
• Decursin inhibited angiogenic and proliferative in HUVEC and HRMVEC in high glucose.
• Decursin reduced VEGFR-2 expression in HUVEC and HRMVEC in excess glucose.
• Decursin reduced VEGFR-2 expression in retina of streptozotocin induced diabetic rat.
• The decursin may be a safe intervention in the treatment of diabetic retinopathy.
Diabetes induces pathologic proliferation and angiogenesis in the retina that leads to catastrophic loss of vision. Decursin is a novel therapeutic that targets the vascular endothelial growth factor (VEGF) receptor (VEGFR) with putative anti-proliferative and anti-angiogenic activities. Thereby we utilized human retinal microvascular endothelial cells (HRMEC) and human umbilical vein endothelial cells (HUVEC) under conditions of excess glucose to explore dose-dependent responses of decursin on markers of migration, angiogenesis, and proliferation. Decursin dose-dependently inhibited tube formation, VEGFR-2 expression, along with relative metabolic activity and 5-bromo-2′-deoxy-uridine (BrdU) activity in both cell lines. We then correlated our findings to the streptozotocin-induced rat model of diabetes. Following three months of decursin treatment VEGFR-2 expression was significantly inhibited. Our data would suggest that decursin may be a potent anti-angiogenic and anti-proliferative agent targeting the VEGFR-2 signaling pathway, which significantly inhibits diabetic retinal neovascularization.
Journal: Molecular and Cellular Endocrinology - Volume 378, Issues 1–2, 25 September 2013, Pages 46–52