کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2196240 | 1098803 | 2013 | 15 صفحه PDF | دانلود رایگان |
Follicle-stimulating hormone (Fsh) function in fishes is poorly understood. This study aimed to reveal Fsh-regulated genes in coho salmon previtellogenic ovarian follicles in vitro. Four suppression subtractive hybridization libraries were generated with RNA isolated from Fsh-treated and control follicles or follicle cell-enriched tissue fractions. Fsh induced steroidogenesis and dynamically upregulated several genes predominantly expressed in follicle cells, including WAP domain-containing protease, connexin 34.3, clusterin (clu1, clu2), fibronectin, wilms tumor 2-like, and influenza virus NS1A-binding protein a. Genes downregulated by Fsh included connective tissue growth factor, alcohol dehydrogenase 8-like, and serine/threonine-protein kinase pim-1. This study demonstrates for the first time in fishes that Fsh influences the expression of a unique suite of ovarian genes involved in processes like cell communication, survival and differentiation, and extracellular matrix remodeling. Collectively, these findings suggest that Fsh and/or steroids induce differentiation of granulosa cells and remodeling of the follicle in preparation for onset of vitellogenesis.
► First transcriptome-wide assessment of ovarian genes regulated by Fsh in fishes.
► Genes expressed in somatic cells of the ovary appear to be most Fsh-responsive.
► Fsh stimulated genes related to cell communication, survival and tissue remodeling.
► Fsh had subtle effects on cell proliferation, differentiation and growth factors.
► Ovarian genes regulated by Fsh in salmon differ in many ways from those in mammals.
► Provides markers for future studies of Fsh function in the fish ovary.
Journal: Molecular and Cellular Endocrinology - Volume 366, Issue 1, 5 February 2013, Pages 38–52