کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2199947 1551178 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular assay optimized by Taguchi experimental design method for venous thromboembolism investigation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Molecular assay optimized by Taguchi experimental design method for venous thromboembolism investigation
چکیده انگلیسی

Two mutations – Factor V Leiden (1691G > A) and the 20210G > A on the Prothrombin gene – are key risk factors for a frequent and potentially fatal disorder called Venous Thromboembolism. These molecular alterations can be investigated using real-time Polymerase Chain Reaction (PCR) with Fluorescence Resonance Energy Transfer (FRET) probes and distinct DNA pools for both factors. The objective of this paper is to present an application of Taguchi Experimental Design Method to determine the best parameters adjustment of a Molecular Assays Process in order to obtain the best diagnostic result for Venous Thromboembolism investigation. The complete process contains six three-level factors which usually demands 729 experiments to obtain the final result, if using a Full Factorial Array. In this research, a Taguchi L27 Orthogonal Array is chosen to optimize the analysis and reduce the number of experiments to 27 without degrading the final result accuracy. The application of this method can lessen the time and cost necessary to achieve the best operation condition for a required performance. The results is proven in practice and confirmed that the Taguchi method can really offer a good approach for clinical assay efficiency and effectiveness improvement even though the clinical diagnostics can be based on the use of qualitative techniques.


► Factor V Leiden and Prothrombin – key risk factors for Venous Thromboembolism.
► Investigation based on PCR with FRET probes and distinct DNA pools for both factors.
► Taguchi Experimental Method – the way for optimizing a Molecular Assays Process.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 25, Issues 5–6, October–December 2011, Pages 231–237
نویسندگان
, , , , , , , ,