کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2202108 1100060 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid modulation of TRH and TRH-like peptide levels in rat brain and peripheral tissues by corticosterone
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Rapid modulation of TRH and TRH-like peptide levels in rat brain and peripheral tissues by corticosterone
چکیده انگلیسی

Disturbance of glucocorticoid signaling has been implicated in several neuropsychiatric disorders including unipolar and bipolar depression and anxiety induced by maternal deprivation. Antidepressants have been shown to be neuroprotective and able to reverse damage to glia and neurons. Thyrotropin-releasing hormone (TRH) is an endogenous antidepressant that reduces the expression of glycogen synthase kinase-3β (GSK-3β), an enzyme that hyperphosphorylates tau and is implicated in bipolar depression, diabetes and Alzheimer's disease. In order to understand the potential role of TRH and TRH-like peptides both as mediators of the depressogenic effects of glucocorticoids and as potential therapeutics for neuropsychiatric disease, 300 g male Sprague-Dawley rats were injected i.p. with 4 mg corticosterone/0.5 ml 50% DMSO + 50% ethanol and sacrificed 0, 2, 4 and 8 h later. Levels of TRH and TRH-like peptides were measured in various brain regions involved in mood regulation and pancreas and reproductive tissues that mediate the metabolic and reproductive impairments associated with high glucocorticoid levels.Significant increases, ranging from 2- to 12-fold, in TRH or TRH-like peptide levels were observed in almost all brain regions studied at 4 h after corticosterone injection. In cerebellum, TRH and TRH-like peptides increased 4–14-fold by 8 h. TRH-like peptide levels fell 86–98% at 4 h after treatment in testis. TRH, derived only from Leydig cells, was not affected. TRH and TRH-like peptides increased 2–4-fold at 8 h in pancreas. TRH and TRH-like peptide concentrations in prostate were not affected by corticosterone up to 8 h after injection. The 4 h needed to detect a highly significant change in the TRH and TRH-like peptide levels in brain and peripheral tissues is consistent with the mediation of most corticosterone-effects via alterations in gene transcription.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 48, Issue 3, February 2006, Pages 208–217
نویسندگان
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