Electroanalytical procedure to resolve a sample solution containing tetracycline and its toxic degraded product: Anhydrotetracycline

The difference in the hydrophobicity of tetracycline and anhydrotetracycline was used to quantify each compound in a mixture. Cyclic voltammetry was employed to characterize the charge transfer processes for tetracycline and anhydrotetracycline across the H2O|1,2-dicloroethane interface. In this paper, we present a comprehensive and detailed study that includes the transfer potential dependence of pH value, the elucidation of the mechanism of transfer using mechanical control of convective flux (N. Wilke, R.A. Iglesias, S.G. Chesniuk, S.A. Dassie, A.M. Baruzzi, Bull. Chem. Soc. Jpn. 75 (2002) 235), and the determination of partition coefficient values employing the change of voltammetric current with external parameters (J.I. Garcia, R.A. Fernández, A.J. Ruggeri, S.A. Dassie, J. Electroanal. Chem. 594 (2006) 80). These studies allow us to introduce an electroanalytical procedure to resolve different mixtures containing tetracycline and anhydrotetracycline in acid degraded tetracycline samples.