کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2204430 | 1100781 | 2014 | 10 صفحه PDF | دانلود رایگان |

• The PCM is not amorphous, but has a defined molecular architecture.
• The PCM comprises proteins organized as molecular fibers and matrices.
• During centrosome maturation the PCM proximal layer acts as a scaffold for PCM expansion.
• 3D volume alignment and averaging determines protein position within few nm error.
• Super-resolution microscopy with quantitative image analysis reveals organelle architecture.
The centrosome influences the shape, orientation and activity of the microtubule cytoskeleton. The pericentriolar material (PCM), determines this functionality by providing a dynamic platform for nucleating microtubules and acts as a nexus for molecular signaling. Although great strides have been made in understanding PCM activity, its diffraction-limited size and amorphous appearance on electron microscopy (EM) have limited analysis of its high-order organization. Here, we outline current knowledge of PCM architecture and assembly, emphasizing recent super-resolution imaging studies that revealed the PCM has a layered structure made of fibers and matrices conserved from flies to humans. Notably, these studies debunk the long-standing view of an amorphous PCM and provide a paradigm to dissect the supramolecular organization of organelles in cells.
Journal: - Volume 24, Issue 3, March 2014, Pages 188–197