کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2204430 1100781 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Amorphous no more: subdiffraction view of the pericentriolar material architecture
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Amorphous no more: subdiffraction view of the pericentriolar material architecture
چکیده انگلیسی


• The PCM is not amorphous, but has a defined molecular architecture.
• The PCM comprises proteins organized as molecular fibers and matrices.
• During centrosome maturation the PCM proximal layer acts as a scaffold for PCM expansion.
• 3D volume alignment and averaging determines protein position within few nm error.
• Super-resolution microscopy with quantitative image analysis reveals organelle architecture.

The centrosome influences the shape, orientation and activity of the microtubule cytoskeleton. The pericentriolar material (PCM), determines this functionality by providing a dynamic platform for nucleating microtubules and acts as a nexus for molecular signaling. Although great strides have been made in understanding PCM activity, its diffraction-limited size and amorphous appearance on electron microscopy (EM) have limited analysis of its high-order organization. Here, we outline current knowledge of PCM architecture and assembly, emphasizing recent super-resolution imaging studies that revealed the PCM has a layered structure made of fibers and matrices conserved from flies to humans. Notably, these studies debunk the long-standing view of an amorphous PCM and provide a paradigm to dissect the supramolecular organization of organelles in cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 24, Issue 3, March 2014, Pages 188–197
نویسندگان
, , , ,