Palladized aluminum electrode covered by Prussian blue film as an effective transducer for electrocatalytic oxidation and hydrodynamic amperometry of N-acetyl-cysteine and glutathione

The mediated oxidation of N-acetyl cysteine (NAC) and glutathione (GL) at the palladized aluminum electrode modified by Prussian blue film (PB/Pd–Al) is described. The catalytic activity of PB/Pd–Al was explored in terms of FeIII[FeIII(CN)6]/FeIII[FeII(CN)6]1− system by taking advantage of the metallic palladium layer inserted between PB film and Al, as an electron-transfer bridge. The best mediated oxidation of NAC and GL on the PB/Pd–Al electrode was achieved in 0.5 M KNO3 + 0.2 M potassium acetate of pH 2. The mechanism and kinetics of the catalytic oxidation reactions of the both compounds were monitored by cyclic voltammetry and chronoamperometry. The charge transfer-rate limiting step as well as overall oxidation reaction of NAC or GL is found to be a one-electron abstraction. The values of transfer coefficients α, catalytic rate constant k and diffusion coefficient D are 0.5, 3.2 × 102 M−1 s−1 and 2.45 × 10−5 cm2 s−1 for NAC and 0.5, 2.1 × 102 M−1 s−1 and 3.7 × 10−5 cm2 s−1 for GL, respectively. The modifying layers on the Pd–Al substrate have reproducible behavior and a high level of stability in the electrolyte solutions. The modified electrode is exploited for hydrodynamic amperometry of NAC and GL. The amperometric calibration graph is linear in concentration ranges 2 × 10−6–40 × 10−6 for NAC and 5 × 10−7–18 × 10−6 M for GL and the detection limits are 5.4 × 10−7 and 4.6 × 10−7 M, respectively.