کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
22121 43255 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Monitoring of Phytopathogenic Ralstonia solanacearum Cells Using Green Fluorescent Protein-Expressing Plasmid Derived from Bacteriophage ϕRSS1
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Monitoring of Phytopathogenic Ralstonia solanacearum Cells Using Green Fluorescent Protein-Expressing Plasmid Derived from Bacteriophage ϕRSS1
چکیده انگلیسی

A green fluorescent protein (GFP)-expressing plasmid was constructed from a filamentous bacteriophage ϕRSS1 that infects the phytopathogen Ralstonia solanacearum. This plasmid designated as pRSS12 (4.7 kbp in size) consists of an approximately 2248 bp region of the ϕRSS1 RF DNA, including ORF1-ORF3 and the intergenic region (IG), and a Km cassette in addition to the GFP gene. It was easily introduced by electroporation and stably maintained even without selective pressure in strains of R. solanacearum of different races and biovars. Strong green fluorescence emitted from pRSS12-transformed bacterial cells was easily monitored in tomato tissues (stem, petiole, and root) after infection as well as from soil samples. These results suggest that pRSS12 can serve as an easy-to-use GFP-tagging tool for any given strain of R. solanacearum in cytological as well as field studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 104, Issue 6, December 2007, Pages 451–456
نویسندگان
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