کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
22173 | 43258 | 2006 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional expression of streptococcal galactosyltransferase in baculovirus/insect cell expression system
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Functional expression of streptococcal galactosyltransferase in baculovirus/insect cell expression system Functional expression of streptococcal galactosyltransferase in baculovirus/insect cell expression system](/preview/png/22173.png)
چکیده انگلیسی
The cpsIaJ gene of Streptococcus agalactiae type Ia codes for β-1,4-galactosyltransferase. In this study, the functional expression of His-tagged CpsIaJ in a baculovirus expression system was performed, because the efficient functional expression of this enzyme in Escherichia coli had been unsuccessful. Using a partially purified enzyme preparation, we found that the enzyme had a restricted substrate specificity and that the entire structure of the substrate GlcNAcβ1-3Galβ1-4Glc was required for the activity. Furthermore, mutations in a conserved DXD motif caused the loss of the enzyme's activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 4, April 2006, Pages 372–375
Journal: Journal of Bioscience and Bioengineering - Volume 101, Issue 4, April 2006, Pages 372–375
نویسندگان
Yohei Kataoka, Shingo Ozeki, Katsuhide Miyake, Shinji Iijima,