Solution-stirring method improves crystal quality of human triosephosphate isomerase

We examined the effect of a solution-stirring method on human triosephosphate isomerase crystallization. The crystals diffracted to more than 1.4 Å resolution, whereas those obtained by the normal vapour-diffusion technique diffracted to 2.8 Å. These results clearly show that the solution-stirring method is valuable and useful for protein crystallization because of its effectiveness and simplicity.