کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
22411 43276 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of HLA-A24-restricted epitopes with high affinities to Hsp70 using peptide arrays
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Identification of HLA-A24-restricted epitopes with high affinities to Hsp70 using peptide arrays
چکیده انگلیسی
Heat shock protein 70 (Hsp70) family members are known as facilitators of immune responses by interacting with receptors on antigen-presenting cells leading to Hsp70-peptide uptake and antigen cross priming. Here, identification of human leukocyte antigen (HLA)-A24-restricted epitopes was achieved using peptide arrays for evaluation of their affinities to Hsp70 and HLA-A24 binding prediction tools. Using Hsp70 as the model antigen, the GYPVTNAVI and VFQHGKVEI peptides were identified as antigens. These peptides actually bound to HLA-A24 in the stabilization assay using T2-A*2402 cells, and induced a strong peptide-reactive cytotoxic T lymphocyte response in HLA-A24 transgenic mice after vaccination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 105, Issue 3, March 2008, Pages 198-203
نویسندگان
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