Extraction of protocatechuic acid from Scutellaria barbata D. Don using supercritical carbon dioxide

⿢The extraction of PA from S. barbata using SC⿿CO2 technique with water is described for the first time.⿢Solubility data of PA in SC⿿CO2 correlated well using the Chrastil model.⿢Optimized conditions resulted in high yield without the use of organic solvents.⿢The SC⿿CO2 method dramatically reduced time and energy consumption.⿢The SC⿿CO2 extract process is an environmentally friendly technology for pharmaceutical industry.

The use of supercritical carbon dioxide (SC⿿CO2), with water as a modifier, was evaluated in this study as a method to extract protocatechuic acid (PA) from Scutellaria barbata D. Don. The highest extraction yield of PA, 64.094 ± 2.756 μg/g of dry plant, was achieved at 75 °C and 27.5 MPa, with the addition of 15.6% (v/v) water as a modifier. The mean particle size was 0.355 mm, the CO2 flow rate was 2.2 mL/min (STP) and the dynamic extraction time was 100 min. At pressures of 16.2⿿30.0 MPa and temperatures of 45⿿75 °C, the mole fraction solubilities of PA in SC⿿CO2 ranged from 2.829 ÿ 10⿿7 to 9.631 ÿ 10⿿7. The solubility data for PA fit well in the Chrastil model. It is evident that the SC⿿CO2 extraction uses less solvent, saves both energy and time and is an environmentally friendly extract technology that can be used in the food, cosmetic and pharmaceutical industries.

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