Lysozyme encapsulation within PLGA and CaCO3 microparticles using supercritical CO2 medium

This study is part of a global project which aims at conceiving and characterizing implantable synthetic extracellular matrices seeded with multifunctional particles for bone and cartilage tissue engineering. To date, protein encapsulation remains a challenge in the field of microencapsulation. The aim of this work is to use supercritical CO2 to generate polymeric and inorganic particles for protein encapsulation. Poly(lactic-co-glycolic) (PLGA) and calcium carbonate (CaCO3) microparticles were chosen to be investigated. In both cases, spherical particles were successfully obtained based on the formation of an emulsion in CO2 media. Most importantly, only non-toxic solvents or aqueous solution were used for the formulation of microparticles. Encapsulation experiments were carried out to provide a proof of concept. Lysozyme was chosen as a model protein and experimental designs were made in order to better understand the system and to better predict the encapsulation yield. The encapsulation yield can reach about 60% in both cases. The mechanism of particle formation and lysozyme encapsulation will also be discussed in detail.

Figure optionsDownload as PowerPoint slideHighlights
► PLGA and CaCO3 microparticles have been successfully formulated using supercritical CO2.
► Non-toxic injectable solvent (glycofurol) was used for the formulation of poly(lactic-co-glycolic) acid PLGA microparticles in CO2 medium.
► Supercritical CO2 process conducted to the formation of porous CaCO3 microparticles.
► Lysozyme has been successfully encapsulated with high encapsulation yields into PLGA and CaCO3 microparticles.