کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
232285 1427479 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Supercritical fluid extraction of antioxidant compounds from oregano: Chemical and functional characterization via LC–MS and in vitro assays
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Supercritical fluid extraction of antioxidant compounds from oregano: Chemical and functional characterization via LC–MS and in vitro assays
چکیده انگلیسی

The possibility of using oregano leaves as a new source of natural antioxidants has been investigated. In the present study, oregano leaves were extracted using a pilot-scale SFE plant under a wide range of extracting conditions (at different extraction pressures and temperatures and considering the addition of ethanol as modifier) with the objective of knowing not only the best extraction conditions but also the variables that control the process in terms of extraction and fractionation of products of high antioxidant activity. The different fractions obtained at each experimental condition tested were characterized in terms of chemical composition by using liquid chromatography–diode array detection (LC–DAD) and liquid chromatography–mass spectrometry (LC–MS). Nine compounds have been tentatively identified as belonging to a specific family of compounds (flavone, flavanone and flavonol); their aglycon structures have been proposed using UV spectra profile while a tentative pattern of substitution, based on their mass spectra and molecular ion (MH+) major fragments, has been also given. Antioxidant activity was measured using two different in vitro assays (1,1-diphenyl-2-picrylhydrazyl (DPPH) antiradical test and bleaching β-carotene method).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Supercritical Fluids - Volume 38, Issue 1, August 2006, Pages 62–69
نویسندگان
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