کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2428501 1106318 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immunogenicity of multiple-epitope antigen gene of HCV carried by novel biodegradable polymers
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Immunogenicity of multiple-epitope antigen gene of HCV carried by novel biodegradable polymers
چکیده انگلیسی
In order to develop a promising vaccine candidate utilizing a combined approach to induce both antibody production and T-cell activity, the DNA fragment containing MA of HCV with five conserved epitopes was synthesized. Two types of HCV vaccine candidates (the DNA type and DNA/polymers) were constructed using MA. PLA-PEG-PLA and PLGA-PEG-PLGA were synthesized and used as micelles with encapsulated plasmid pcDNA3.1(+)-MA. The preparation of copolymers, the cloning and analysis of recombinant plasmid DNA, in vitro expression, and immunogenicity in transgenic mice were evaluated in detail. The results indicated that even single immunization and oral immunization with DNA/polymers achieved satisfying immune responses in vivo tests. As biodegradable and nontoxic triblock copolymers, the novel copolymers demonstrated a great advantage, as they made long-term and single-immunizing vaccines possible; in addition, the copolymers showed a better adjuvant effect and scarcely any side effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Immunology, Microbiology and Infectious Diseases - Volume 34, Issue 1, January 2011, Pages 65-72
نویسندگان
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