کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2429065 1553579 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
A NK-lysin from Cynoglossus semilaevis enhances antimicrobial defense against bacterial and viral pathogens
چکیده انگلیسی


• A NK-lysin gene, CsNKL1, was identified in half-smooth tongue sole.
• CsNKL1 contains five exons and four introns.
• CsNKL1 was expressed in multiple tissues and induced by pathogen infection.
• Overexpression of CsNKL1 in tongue sole stimulated immune gene expression.
• Overexpression of CsNKL1 inhibits bacterial and viral infection.

NK-lysin is an effector protein of cytotoxic T lymphocytes and natural killer cells. Mammalian NK-lysin is known to possess antibacterial property and antitumor activity. Homologues of NK-lysin have been identified in several teleost species, but the natural function of fish NK-lysin remains essentially unknown. In this study, we identified a NK-lysin, CsNKL1, from half-smooth tongue sole (Cynoglossus semilaevis) and analyzed its expression, genetic organization, and biological effect on pathogen infection. CsNKL1 is composed of 135 residues and shares 33.1–56.5% overall sequence identities with other teleost NK-lysin. CsNKL1 possesses a Saposin B domain and six conserved cysteine residues that in mammals are known to form three intrachain disulfide bonds essential to antimicrobial activity. The genomic sequence of the ORF region of CsNKL1 is 1240 bp in length and, like human NK-lysin, contains five exons and four introns. Expression of CsNKL1 occurred in multiple tissues and was upregulated by bacterial and viral infection in a time dependent manner. When CsNKL1 was overexpressed in tongue sole, significant upregulation of interleukin-1 and chemokines was observed in spleen and head kidney. Following bacterial and viral infection, the pathogen loads in the tissues of CsNKL1-overexpressing fish were significantly lower than those in control fish. These results indicate that CsNKL1 possesses the novel capacities of immunomodulation and enhancing antimicrobial defense against pathogens of both bacterial and viral nature.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 40, Issues 3–4, July–August 2013, Pages 258–265
نویسندگان
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