کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2429221 | 1106485 | 2013 | 16 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish Phylogenetic analysis of vertebrate CXC chemokines reveals novel lineage specific groups in teleost fish](/preview/png/2429221.png)
• An in depth analysis of the phylogeny of vertebrate CXC chemokines was undertaken.
• A nomenclature for fish specific CXC chemokines have been proposed.
• IL-1β, type I and II IFN differentially modulate trout CXC chemokine expression.
• The chemoattractant activity of three of the trout CXC chemokines was studied.
In this study, we have identified 421 molecules across the vertebrate spectrum and propose a unified nomenclature for CXC chemokines in fish, amphibians and reptiles based on phylogenetic analysis. Expanding on earlier studies in teleost fish, lineage specific CXC chemokines that have no apparent homologues in mammals were confirmed. Furthermore, in addition to the two subgroups of the CXCL8 homologues known in teleost fish, a third group was identified (termed CXCL8_L3), as was a further subgroup of the fish CXC genes related to CXCL11. Expression of the CXC chemokines found in rainbow trout, Oncorhynchus mykiss, was studied in response to stimulation with inflammatory and antiviral cytokines, and bacterial. Tissue distribution analysis revealed distinct expression profiles for these trout CXC chemokines. Lastly three of the trout chemokines, including two novel fish specific CXC chemokines containing three pairs of cysteines, were produced as recombinant proteins and their effect on trout leucocyte migration studied. These molecules increased the relative expression of CD4 and MCSFR in migrated cells in an in vitro chemotaxis assay.
Journal: Developmental & Comparative Immunology - Volume 41, Issue 2, October 2013, Pages 137–152