کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2429230 1106485 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Expressions of transcription factors in goldfish (Carassius auratus L.) macrophages and their progenitors
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Expressions of transcription factors in goldfish (Carassius auratus L.) macrophages and their progenitors
چکیده انگلیسی


• The expression of transcription factors (TF) was measured in macrophage cultures.
• TF mRNA levels of irf8, runx1 and pu.1 were higher in monocytes compared to other cells.
• The mRNA levels of TF associated with erythropoiesis decreased in macrophage cultures.
• Myeloid TF in sorted progenitor cells were modulated by colony-stimulating factor-1 and kit ligand A.

The development of macrophages is a highly regulated process requiring coordination amongst transcription factors. The presence/absence, relative levels, antagonism, or synergy of all transcription factors involved is critical to directing lineage cell fate and differentiation. While relative levels of many key myeloid transcription factors have been determined in mammalian macrophage differentiation, a similar set of studies have yet to be conducted in a teleost system. In this study, we report on the mRNA levels of transcription factors (cebpa, cjun, cmyb, egr1, gata1, gata2, gata3, lmo2, mafb, pax5, pu.1 and runx1) in sorted goldfish progenitor cells, monocytes, and macrophages from primary kidney macrophage cultures. The mRNA levels of runx1 and pu.1 were significantly higher, gata3 and pax5 mRNA levels were lower, in monocytes compared to progenitors, and the mRNA levels of cjun, egr1, gata2, gata3, mafb and pax5 were significantly decreased in macrophages compared to progenitor cells. The relative mRNA levels of the interferon regulatory factor family of transcription factors, irf1, irf2, irf5, irf7, irf8 and irf9 in sorted progenitors, monocytes and macrophages were also measured. In contrast to other irf family transcription factors examined, irf8 mRNA levels were increased in monocytes compared to progenitors by greater than three-fold, suggesting that irf8 is important for monopoiesis. Lastly, we show the differential regulation of myeloid transcription factor mRNA levels in sorted progenitor cells from 1, 2, or 3-day old cultures in response to the recombinant goldfish growth factors, rgCSF-1 and rgKITLA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 41, Issue 2, October 2013, Pages 230–239
نویسندگان
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