کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2429383 1106493 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Enzyme characterisation and gene expression profiling of Atlantic salmon chicken- and goose-type lysozymes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Enzyme characterisation and gene expression profiling of Atlantic salmon chicken- and goose-type lysozymes
چکیده انگلیسی

Lysozymes represent important innate immune components against bacteria. In this study, Atlantic salmon (Salmo salar) goose (g-) and chicken (c-) types of lysozyme were subjected to protein characterisations and tissue expression analyses. Specific bacterial protein inhibitors of g- and c-type lysozymes were employed to discriminate between respective enzyme activities. Blood, gills and liver contained activities exclusive for the g-type lysozyme. Only haematopoietic organs (head kidney and spleen) contained enzyme activities of both g- and c-lysozyme enzymes and c-type activity was not found outside these organs. Gene transcript levels proportional to enzyme activity levels were detected for the g-type lysozyme but not for the c-type. In vitro studies revealed significant induction of c-type gene expression and enzyme activity in macrophages after incubation with lipopolysaccharide (LPS) while expression of the g-type lysozyme gene was unaffected. The activity of purified native c-type enzyme was profoundly reduced by divalent cations and displayed low tolerance to monovalent cations, while the native g-type lysozyme was stimulated by monovalent cations and tolerated low concentrations of divalent cations. Activities of both enzymes increased with temperature elevations up to 60 °C. The native g-type lysozyme responses to temperature in particular are in apparent conflict to the ones for the recombinant salmon g-lysozyme.Our results imply separate expression regulations and different functions of c- and g-type lysozymes in salmon. LPS-induced expression of c-type lysozyme and broad constitutive tissue distribution of g-type lysozyme in salmon is different from findings in other studied fish species.


► First time isolation and characterisation of native salmon c- and g-type lysozymes.
► Native c-type lysozyme activity is very sensitive to cations.
► g-type lysozyme activities and gene transcripts found in most tissues.
► Constitutive c-type lysozyme activities only found in haematopoietic tissues.
► Different from other fish species: expression of c-type but not g-type induced by LPS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 40, Issue 1, May 2013, Pages 11–19
نویسندگان
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