Cross-species association of quail invariant chain with chicken and mouse MHC II molecules

There are different degrees of similarity among vertebrate invariant chains (Ii). The aim of this study was to determine the relationship between quail and other vertebrate Ii MHC class II molecules. The two quail Ii isoforms (qIi-1, qIi-2) were cloned by RACE, and qRT-PCR analysis of different organs showed that their expression levels were positively correlated with MHC II gene (B-LB) transcription levels. Confocal microscopy indicated that quail full-length Ii co-localized with MHC II of quail, chicken or mouse in 293FT cells co-transfected with both genes. Immunoprecipitation and western blotting further indicated that these aggregates corresponded to polymers of Ii and MHC class II molecules. This cross-species molecular association of quail Ii with chicken and mouse MHC II suggests that Ii molecules have a high structural and functional similarity and may thereby be used as potential immune carriers across species.

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► Quail Ii isoforms (qIi-1, qIi-2) were cloned by RACE.
► qii-1, qIi-2, and qB-LB transcription levels were positively correlated in different organs.
► Quail Ii and MHC class II molecules of quail, chicken and mouse co-localized in transfected cells.
► Quail Ii bound to MHC class II molecules of quail, chicken, and mouse by immunoprecipitation.