کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2429582 1553581 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The bovine genomic DNA sequence data reveal three IGHV subgroups, only one of which is functionally expressed
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
The bovine genomic DNA sequence data reveal three IGHV subgroups, only one of which is functionally expressed
چکیده انگلیسی

A comprehensive analysis of cattle shotgun sequencing data reveals 36 immunoglobulin heavy chain variable genes. The previously described bovine subgroup IGHV1 contains 10 functional genes with a conserved promoter including the consensus octamer and several other transcription factor binding sites, intact exons and matching cDNA sequences. Subgroups IGHV2 and IGHV3 consist entirely of pseudogenes. Thus, the bovine germline IGHV repertoire is very limited. The IGHV genes are distributed in mammalian clans I and II, while no clan III genes were detected. Clan-specific PCR of genomic DNA from cattle, sheep, Eurasian elk, white-tailed deer, pig and dolphin indicates highly dynamic evolution of IGHV gene usage within Cetartiodactyla. The bovine germline IGHV repertoire was probably generated by recent duplications of an IGHV1-IGHV2 homology unit. Immunoglobulin heavy chain genes are largely incorrectly assembled in the current cattle genome versions Btau_4.2 and UMD_3.1. FISH experiments confirm an IGHV locus close to terminus of BTA21.


► The bovine immunoglobulin heavy chain variable (IGHV) gene repertoire was characterized based on genome sequencing data.
► The bovine genome reveals 36 IGHV genes, distributed in three subgroups in mammalian clans I and II.
► The bovine IGHV repertoire is very limited, with only 10 functional genes revealed in the genome sequence data.
► The evolution of IGHV gene clan usage is highly dynamic within Cetartiodactyla.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 37, Issues 3–4, July 2012, Pages 457–461
نویسندگان
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