کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2429648 1106511 2012 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Time course proteomic profiling of cellular responses to immunological challenge in the sea urchin, Heliocidaris erythrogramma
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Time course proteomic profiling of cellular responses to immunological challenge in the sea urchin, Heliocidaris erythrogramma
چکیده انگلیسی

Genome sequences and high diversity cDNA arrays have provided a detailed molecular understanding of immune responses in a number of invertebrates, including sea urchins. However, complementary analyses have not been undertaken at the level of proteins. Here, we use shotgun proteomics to describe changes in the abundance of proteins from coelomocytes of sea urchins after immunological challenge and wounding. The relative abundance of 345 reproducibly identified proteins were measured 6, 24 and 48 h after injection. Significant changes in the relative abundance of 188 proteins were detected. These included pathogen-binding proteins, such as the complement component C3 and scavenger receptor cysteine rich proteins, as well as proteins responsible for cytoskeletal remodeling, endocytosis and intracellular signaling. An initial systemic reaction to wounding was followed by a more specific response to immunological challenge involving proteins such as apolipophorin, dual oxidase, fibrocystin L, aminopeptidase N and α-2-macroglobulin.

Figure optionsDownload high-quality image (142 K)Download as PowerPoint slideHighlights
► We described the proteome of sea urchins after wounding and challenge with lipopolysaccharides.
► Shotgun proteomics was powerful in identifying and quantifying 345 proteins.
► We observed changes 6, 24 and 48 h after injection.
► An initial systemic reaction to wounding is followed by a more specific response to immunological challenge.
► Immune response to LPS involves apolipophorin, dual oxidase, fibrocystin L, aminopeptidase N and α-2-macroglobulin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 37, Issue 2, June 2012, Pages 243–256
نویسندگان
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