کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2429740 | 1106518 | 2009 | 12 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties](/preview/png/2429740.png)
A cluster of 11 interferon (IFN) genes were identified in the Atlantic salmon genome linked to the growth hormone 1 gene. The genes encode three different IFN subtypes; IFNa (two genes), IFNb (four genes) and IFNc (five genes), which show 22–32% amino acid sequence identity. Expression of the fish IFNs were studied in head kidney, leukocytes or TO cells after stimulation with the dsRNA poly I:C or the imidazoquinoline S-27609. In mammals, poly I:C induces IFN-β through the RIG-I/MDA5 or the TLR3 pathway, both of which are dependent on NF-kB. In contrast, S-27609 induces mammalian IFN-α in plasmacytoid dendritic cells through the TLR7 pathway independent of NF-κB. The presence of an NF-κB site in their promoters and their strong up-regulation by poly I:C, suggest that salmon IFNa1/IFNa2 are induced through similar pathways as IFN-β. In contrast, the apparent lack of NF-κB motif in the promoter and the strong upregulation by S-27609 in head kidney and leukocytes, suggest that IFNb genes are induced through a pathway similar to mammalian IFN-α. IFNc genes showed expression patterns different from both IFNa and IFNb. Taken together, salmon IFNa and IFNb are not orthologs of mammalian IFN-β and IFN-α, respectively, but appear to utilize similar induction pathways.
Journal: Developmental & Comparative Immunology - Volume 33, Issue 4, April 2009, Pages 547–558