کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2429801 1106522 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The possible role of penaeidin5 from the black tiger shrimp, Penaeus monodon, in protection against viral infection
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
The possible role of penaeidin5 from the black tiger shrimp, Penaeus monodon, in protection against viral infection
چکیده انگلیسی

Penaeidin class 5 (PEN5) has so far only been reported in the Chinese shrimp, Fenneropenaeus chinensis, and the black tiger shrimp, Penaeus monodon. The PEN5 homolog from F. chinensis (FenchiPEN5) exhibits antimicrobial activities against both Gram-positive and Gram-negative bacteria as well as fungi. Here, we characterized the PEN5 gene from P. monodon (PenmonPEN5) and evaluated its potential involvement in antiviral immunity. The deduced open reading frame of PenmonPEN5 encodes for a predicted 79 amino acid peptide including a 19 amino acid signal peptide. The gene structure of PenmonPEN5 contains two exons interrupted by one intron, whilst the 5′ upstream sequence contains a putative TATA box and several GATA, GATA-3, AP-1 and dorsal transcription factor binding sites. PenmonPEN5 mRNA levels in P. monodon shrimps following a systemic infection with white spot syndrome virus (WSSV) were significantly induced at 24 h post infection, but was strongly down-regulated at 48 h post injection, compared to those of the uninfected control shrimps. The suppression of PenmonPEN5 transcript levels by RNA interference mediated gene silencing led to an increased susceptibility of shrimps to WSSV infection, suggesting a possible role of PenmonPEN5 in the shrimp's antiviral immunity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 35, Issue 5, May 2011, Pages 530–536
نویسندگان
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