کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2430104 1106542 2008 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Natural killer cells in lymph nodes of healthy calves express CD16 and show both cytotoxic and cytokine-producing properties
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Natural killer cells in lymph nodes of healthy calves express CD16 and show both cytotoxic and cytokine-producing properties
چکیده انگلیسی

SummaryNatural killer (NK) cells were recently shown to play an important immunomodulatory role in lymph nodes. We here report the presence, phenotype and function of NK cells resident in lymph nodes of several anatomical sites of healthy calves. NKp46+/CD3−lymphocytes, recently demonstrated to precisely identify NK cells in all tested species, were present in the paracortex and the medulla of bovine lymph nodes. Most lymph node-derived NK cells expressed CD16 and perforin, and a lytic capacity was demonstrated, while a well-developed interferon-gamma response to interleukin-2 and interleukin-12 stimulation was also seen. Lymph node-derived NK cells differed from those in blood by a higher expression of the activation markers CD44 and CD25, as well as CD8. L-selectin (CD62L) was expressed by the majority of lymph node-derived NK cells, consistent with a dependency of this molecule for migration to lymph nodes. Unlike in blood, the majority of lymph node NK cells had little or no CD2 expression. Compared to available literature, calf lymph nodes contained NK cells in numbers equal to or higher than reported in humans, and clearly higher than in mice. These findings suggest a cytotoxic role of lymph node residing NK cells, beyond the predominantly cytokine-producing role previously inferred from studies on human NK cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 32, Issue 7, 2008, Pages 773–783
نویسندگان
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