کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2430223 1106552 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gene expression profiling of chicken lymphoid cells after treatment with Lactobacillus acidophilus cellular components
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Gene expression profiling of chicken lymphoid cells after treatment with Lactobacillus acidophilus cellular components
چکیده انگلیسی

Lactobacillus acidophilus has been shown to exert immunostimulating activities in a number of species, including the chicken. To examine the molecular mechanisms of this phenomenon, we investigated spatial and temporal expression of immune system genes in chicken cecal tonsil and spleen mononuclear cells in response to structural constituents of L. acidophilus. Using a low-density chicken immune system microarray, we found that cecal tonsil cells responded more rapidly than spleen cells to the bacterial stimuli, with the most potent stimulus for cecal tonsil cells being DNA and for splenocytes being the bacterial cell wall components. We also discovered that in both splenocytes and cecal tonsil cells, STAT2 and STAT4 genes were highly induced. Given the close interactions between cecal tonsil cells and commensal bacteria, we further examined the involvement of STAT2 and STAT4 signaling pathways in cellular responses to bacterial DNA. Our results revealed that the expression of STAT2, STAT4, IL-18, MyD88, IFN-α, and IFN-γ genes were up-regulated in cecal tonsil cells after treatment with L. acidophilus DNA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 32, Issue 5, 2008, Pages 563–574
نویسندگان
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