کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2430262 1106555 2008 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cytokines and chemokines in postovulatory follicle regression of domestic chicken (Gallus gallus domesticus)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
Cytokines and chemokines in postovulatory follicle regression of domestic chicken (Gallus gallus domesticus)
چکیده انگلیسی

The mechanism of postovulatory follicle (POF) regression in birds is still poorly understood. In the current study, expression of IL-1β, IL-6, GM-CSF, IFN-γ, IL-2, IL-4, IL-13, chCXCLi2, chCCLi2, chCCLi4, chCCLi7, IL-10 and TGF-β2 mRNAs was estimated in regressing POF by semi-quantitative RT-PCR. In addition, the changes in immune cell population, histological and apoptotic changes were also studied in regressing POF. The expression of cytokines (IL-1β, IL-6, IL-10 and TGF-β2) and chemokines (chCXCLi2, chCCLi2, chCCLi4 and chCCLi7) was upregulated in POFs, suggesting a role for these molecules in tissue regression. The histological findings suggested a significant infiltration of immune cells, especially heterophils, lymphocytes and macrophages, into the regressing POF. The flow cytometry analysis of lymphocyte subpopulations revealed that CD3+, CD4+, CD8+ and Bu-1+ lymphocytes were significantly increased during this regression. The significant up-regulation of chemokines might have attracted the immune cells during POF regression. The percentage of apoptotic cells was significantly increased during the regression of POF. The up-regulation of IL-1β, IL-6, IL-10 and TGF-β2 and down-regulation of GM-CSF might have induced apoptosis during the POF regression. However, expression of IFN-γ, IL-2, IL-4 and IL-13 was not significantly altered during POF regression. In conclusion, cytokines appear to play an important role in the regression of POF in chicken. Furthermore, the regression of chicken POF seems to be an inflammatory event similar to luteolysis of the mammalian corpus luteum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 32, Issue 3, 2008, Pages 253–264
نویسندگان
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